Antifeedant Activity of Ethanolic Leaf Extract of Lantana camara Against Crocidolomia pavonana and Spodoptera litura
Abstract
Chemical insecticides that have been intensively used to control cabbage caterpillars have become the main causes of pest resistance, high chemical residues in crop products and the decrease of biodiversity of natural enemies in the environment. Secondary metabolites from plant extract provide opportunities to be developed as bioinsecticides. The objective of this research was to evaluate the antifeedant effects of ethanolic leaf extract of L. camara on Crocidolomia pavonana and Spodoptera litura larvae for pre eliminary study. The crude extract was prepared by leaf maceration of L. camara leaf using ethanol, evaporation of the solvent, followed by antifeedant bioassay test. Leaf disc dipping method with choice and no choice tests used to find out the effective minimum concentration on feeding deterrent activity. The mean leaf area consumed were analyzed by using nonparametric statistics of Mann-Whitney U. The results showed that the minimum effective concentration of ethanolic leaf extract that deter the feeding activity of C. pavonana larvae was 2000 ppm (choice test) and 1000 ppm (no choice test) (P<0.05) and consider as medium antifeedant category. The minimum concentration showing antifeedant activity to S. litura larvae was 500 ppm (choice test) but result no choice test was showed not significant antifeedant activity (P>0.05). This performance consider as medium antifeedant category on S.litura larvae at 500 ppm, in concentrations 1000ppm-5000ppm, the extract of L. camara were tended as attractant on S.litura larvae.
References
Rauf, A.; Prijono, D.; Russell, D. A. Survey of pest control practices of cabbage farmers in West Java , Indonesia SURVEY OF PEST CONTROL PRACTICES OF CABBAGE FARMERS IN WEST JAVA , INDONESIA Department of Plant Pests and Diseases , Bogor Agricultural University , Centre for Environmental Str. 2004, doi:10.13140/RG.2.2.28292.83840.
Zhang, M.; Zeiss, M. R.; Geng, S. Agricultural pesticide use and food safety: California’s model. J. Integr. Agric. 2015, 14, 2340–2357, doi:10.1016/S2095-3119(15)61126-1.
Baskar, K.; Maheswaran, R.; Pavunraj, M.; Packiam, S. M.; Ignacimuthu, S.; Duraipandiyan, V.; Benelli, G. Toxicity and antifeedant activity of Caesalpinia bonduc (L.) Roxb. (Caesalpiniaceae) extracts and fractions against the cotton bollworm Helicoverpa armigera Hub. (Lepidoptera: Noctuidae). Physiol. Mol. Plant Pathol. 2018, 101, 69–74, doi:10.1016/j.pmpp.2017.01.006.
Paul, D.; Choudhury, M. Larvicidal and antifeedant activity of some indigenous plants of Meghalaya against 4 th instar Helicoverpa armigera ( Hübner ) larvae. 2016, 5, 447–460, doi:10.18869/modares.jcp.5.3.447.
Kalita, S.; Kumar, G.; Karthik, L.; Rao, K. V. B. A review on medicinal properties of lantana camara linn. Res. J. Pharm. Technol. 2012, 5, 711–715, doi:10.1155/2016/4104595.
Sousa, E. O.; Costa, J. G. M. Genus Lantana : chemical aspects and biological activities. 2012, 1155–1180.
Mamta, S.; Jyoti, S. Phytochemical screening Acorus Calamus and Lantana Camara. Pharmacy 2012, 3, 324–326, doi:10.3109/00016358709096356.
Dua, V. K.; Pandey, A. C.; Dash, A. P. Adulticidal activity of essential oil of Lantana camara leaves against mosquitoes. Indian J. Med. Res. 2010, 131, 434–439.
Kayesth, S.; Gupta, K. K. Impact of Lantana camara hexane extract on survival, growth and development of Dysdercus koenigii Fabricius (Heteroptera: Pyrrhocoriedae). Acta Ecol. Sin. 2018, 38, 187–192, doi:https://doi.org/10.1016/j.chnaes.2017.12.002.
Yuan, Z.; Hu, X. P. Repellent, Antifeedant, and Toxic Activities of <I>Lantana camara</I> Leaf Extract Against <I>Reticulitermes flavipes</I> (Isoptera: Rhinotermitidae). J. Econ. Entomol. 2012, 105, 2115–2121, doi:10.1603/EC12026.
Pung, T.; Srimongkolchai, W. Toxic effects of Lantana camara crude extracts on Spodoptera litura (Fabr.). Asian J. Chem. 2011, 23, 2863–2865.
Khaidir PENGUJIAN PENGHAMBATAN AKTIVITAS MAKAN DARI EKSTRAK DAUN Lantana camara L. (Verbenaceae) TERHADAP LARVA Plutella xylostella L. (Lepidoptera: Yponomeutidae) Evaluation. J. Floratek 2013, 8, 35–44.
Mkindi, A.; Mpumi, N.; Tembo, Y.; Stevenson, P. C.; Ndakidemi, P. A.; Mtei, K.; Machunda, R.; Belmain, S. R. Invasive weeds with pesticidal properties as potential new crops. Ind. Crops Prod. 2017, 110, 113–122, doi:10.1016/j.indcrop.2017.06.002.
Dono, D.; Ismayana, S.; Idar; Prijono, D.; Muslikha, I. Status and Biochemical Resistance Of Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) to Organophosphate Insecticide and Its Sensitivity to Botanical Insecticide. J. Entomol. Indon 2010, 7, 9–27, doi:10.5994/jei.7.1.9-27.
Isman, M. Insect antifeedants. Pestic. Outlook 2002, 13, 152–157, doi:10.1039/b206507j.
Hermawan, W.; Kasmara, H.; Melanie; Panatarani, C.; Joni, I. M. Recent advances of rearing cabinet instrumentation and control system for insect stock culture. AIP Conf. Proc. 2017, 1801, doi:10.1063/1.4973103.
Baratella, V.; Pucci, C.; Paparatti, B.; Speranza, S. Response of Bactrocera oleae to different photoperiods and temperatures using a novel method for continuous laboratory rearing. Biol. Control 2017, 110, 79–88, doi:10.1016/j.biocontrol.2017.04.010.
Kasmara, H.; Melanie; Nurfajri, D. A.; Hermawan, W.; Panatarani, C. The toxicity evaluation of prepared Lantana camara nano extract against Spodoptera litura (Lepidoptera: Noctuidae). AIP Conf. Proc. 2018, 1927, doi:10.1063/1.5021239.
Gabrys, B.; Szczepanik, M.; Dancewicz, K.; Szumny, A.; Wawrzeńczyk, C. Environmentally Safe Insect Control : Feeding Deterrent Activity of Alkyl-Substituted γ- and δ ‑ Lactones to Peach Potato Aphid ( Myzus persicae [ Sulz .]) and Colorado Potato Beetle ( Leptinotarsa decemlineata Say ). 2006, 15, 549–556.
Juárez, Z.N., Fortuna, A.M., Arreolac, E.S., López, J.F., Bache H., and Hernández L.R., Antifeedant and Phagostimulant Activity of Extracts and Pure Compounds from Hymenoxys robusta on Spodoptera exigua (Lepidoptera: Noctuidae) Larvae. Natural Product Communications. 2014, 9, 895-898.
Bergaoui, A.Z., Hammami, S., Halima-Kamel, M.B., Sakka-Rouis, L., Boussaada, O., Haouas, D.,and Mighri, Z., Insecticidal Activities of Flowerheads of Anacyclus cyrtolepidioides Pomel Growing in Tunisia Against Tribolium confusum du Val. J. Entomol.Tun. 2008, 5, 277-283.
Schoonhoven, L. M.; Loon, J. J. A. van.; Dicke, M. Insect-plant biology; Oxford University Press, 2005; ISBN 9780198525950.
Koul, O., 2005. Insect Antifeedants. CRC Press, Boca Raton.
